Urease 's an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: » (NH2)2CO + H2O → CO2 + 2NH3 In 1926 James Sumner showed that urease's a protein. Urease's found in bacteria, yeast and several higher plants. Characteristics Active site metal: nickel(II) Molecular weight: 480 kDa or 545 kDa for Jack Bean Urease (calculated mass from the amino acid sequence). Optimum pH: 7.4 Optimum Temperature: 60 degrees Celsius Enzymatic speci… (More on Urease)